This is an unabridged, spiral-bound versions of „Cell Culture Volume 58 of Methods in Enzymology“, designed to meet the needs of the biochemically oriented biologist. It serves both as an instructional source for the novice and as a reference source for more specialized methods. It covers basics in great detail, and laboratory requirements and media cell culture techniques: Monolayers, Growth Measurement, and Mass Culture. It includes specialized techniques, synchroniation, autoradiography, virus preparation cell fusion, mutant isolation, karyotyping, nude mice. It provides examples for propogating special cell types – Stable Cell Lines, Clinical Material, Mammalian Vertebrate, Invertebrate, and Plant Cells; and means for selecting specific cell types.